Analysis Note

ControlBrain tissue

Application

Research CategoryNeuroscience

Immunohistochemistry(paraffin) Analysis:
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum
Immunohistology on frozen sections at 1:10

Western Blot Analysis:
A previous lot of this antibody was used in Western Blot.

ELISA:
A 1:200-1:1,000 dilution of a previous lot was used in ELISA.

RIA:
A previous lot of this antibody was used in Radioimmunoassay.

Optimal working dilutions must be determined by end user.

This Anti-Myelin Basic Protein Antibody, a.a. 129-138, clone 1 is validated for use in ELISA, IH, IH(P), RIA, WB for the detection of Myelin Basic Protein.

Research Sub CategoryNeuronal & Glial MarkersNeurochemistry & Neurotrophins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The classic group of MBP isoforms (isoforms 4-14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoforms 1-3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.

MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.

Isoform 1 Golli-MBP1, HOG7, 33 kDa

Isoform 2 Golli-MBP2, HOG5, 21.5 kDa

Isoform 3 MBP1, 21.5 kDa

Isoform 4 MBP2, 20.2 kDa

Isoform 5 MBP3, 18.5 kDa

Isoform 6 MBP4, 17.2 kDa

(SP_P02686)

Immunogen

Epitope: a.a. 129-138

Bovine myelin basic protein

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Culture supernatant containing 0.2 M Tris/HCl, pH 7.4 with 5-10% fetal calf serum and 0.1% sodium azide

Unpurified

Quality

Routinely evaluated by immunohistochemistry on brain tissue.

Immunohistochemistry(paraffin) Analysis:
MBP (cat. # MAB382) staining pattern/morphology in rat cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:50, using IHC-Select™ Detection with HRP-DAB. Immunoreactivity is seen as fiber staining in the junction between granular layer and molecular layer.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Rat Cerebellum

Specificity

Reacts with MBP from human, bovine and rat, epitope 129-138

Storage and Stability

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

19 kDa